Shows the dental student how to prepare slides and use the microscope in showing a patient the bacteria of their plaque also explains the different bacteria on the slide. Common opportunistic infection from yeast-like organism candida albicans skip to main content menu published by creamy-white soft plaques that can be wiped away to expose an erythematous mucosa (fig 1) cytologic smear samples should be prepared chairside, and then submitted to a laboratory for microscopic diagnosis. My wife's pap smear came up clean so, i guess it's not the any of the usual suspects (yeast, etc) i keep it cleaner than ever but, it still gets gooey and smells btw i am circumsized. Candidiasis yeast infection symptoms and signs signs and symptoms of a candida infection can vary depending on the location of the infection in women , signs and symptoms of a vaginal yeast infection are a white discharge that is thick and often described as having a cottage cheese appearance.
A streak plate involves the progressive dilution of an inoculum of bacteria or yeast over the surface of solidified agar medium in a petri dish the result is that some of the colonies on the plate grow well separated from each other. Read this essay on microbiology come browse our large digital warehouse of free sample essays the yeast cells were easily identified using the indirect method however the cells in the plaque smear were not there was a large area of dye in one area on the slide and did not appear to surround. F describe what you observed in your plaque smear wet mount, direct stained slide and indirectly stained slide what were the similarities what were the differences h describe what you observed in your yeast wet mount, direct stained slide and indirectly stained slide what were the similarities what were the differences best answer. Micro baterial morphology lab or any similar topic specifically for you chains of both cocci and bracillus cells were both visible and identifiable the chains varied in length the cheek and yeast smear was clearer were the cell types the same in all three specimen sets: yeast, plaque, and cheek how were they similar how were they.
The yeast and cheek slides will have some nucleated cells the cheek smear should also have a variety of smaller, non-nucleated bacterial cells of different shapes the plaque slide will have a variety of bacterial cells similar to those seen on the cheekslide. Oral candidiasis, also known as oral thrush among other names, is candidiasis that occurs in the mouth that is, oral candidiasis is a mycosis (yeast/fungal infection) of candida species on the mucous membranes of the mouth. For a wet mount, scrapings or smears obtained from skin, nails, or oral or vaginal mucosa are examined under the microscope for hyphae, pseudohyphae, or budding yeast cells a potassium hydroxide smear, gram stain, or methylene blue is useful for direct demonstration of fungal cells. Laboratory exercise 2: permanent stained smears a objectives 1 compare and contrast the iron-hematoxylin and trichrome stains 2 correctly perform the trichrome stain on fecal material 3 using appropriate technique, examine permanently stained smears, including acid-fast for cryptosporidium if the culture is a yeast, scrape a small. D explain the similarities and differences between the cells in the plaque and those in the yeast smears e describe the size and shape of the types of cells that you were able to see in the smear.
Microbiology microbiology a list three reasons why you might choose to stain a particular slide rather than view it as a. Difference between direct and indirect staining what is the difference between direct and indirect staining d what is heat fixing e why is it necessary to ensure that your specimens are completely air dried prior to heat fixing f describe what you observed in your plaque smear wet-mount, direct stained slide, and indirectly stained. Bacteria morphology essay sample abstract – the purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. The pap smear, although specific, is insensitive, with positive results in only about 25 percent of patients with culture-positive symptomatic vulvovaginal candidiasis14 a rapid yeast detection. A gram-stained smear will show an altered vaginal flora, one filled with pleomorphic gram-negative to gram-variable rods and lacking in the normally present gram-positive lactobacilli a positive whiff test (mixing vaginal secretions with a 10 percent koh solution and noting a fishy smell) and a ph 45 on the vaginal secretion lead to a.
Estrogen, cholesterol, plaque, and atherosclerosis posted on june 4, 2015 by admin during the last few blogs we talked about the relationship between estrogen and one form of heart disease called atherosclerosis. Bacterial morphology study play check smear 100x direct stain yeast-100x direct stain plaque smear-100x direct stain check smear 40x- indirect stain yeast- indirect stain 100x plaque smear-100x indirect stain list three reasons why you might choose to stain a particular slide rather than view it as a. Papules, scales, plaques and eruptions scalp, hair and nails sexually transmitted diseases (stds) a pap smear (pap test) is a medical procedure to screen for abnormal cells of the cervix vaginal yeast infections in women are caused by an organism called candida albicans symptoms of a vaginal yeast infection include vaginal pain with. Since staining of bacterial cells is relatively fast, inexpensive, and simple, it is the most commonly used technique to visualize bacterial cells staining not only makes bacteria more easily seen, but it allows their morphology (eg size and shape) to be visualized more easily.
Difference in plaque smear and yeast smear a wet mount can detect trichomonas, a parasite that causes trichomoniasis, a sexually transmitted disease. Yeast, a type of fungi (plural for fungus), is found in many places from nature, to research labs and even everyday kitchens for baking yeast colonies generally look similar to bacterial colonies some species, such as candida , can grow as white patches with a glossy surface. Plaque can build up for many years without symptoms, eventually causing a heart attack or stroke high blood pressure, diabetes, and smoking can all cause plaque to build up, too.
Yeast smear don’t contain long bacillus, while plaque smear contains them e: the cheek smear cells have irregular borders, and they are mostly round in the shape some of them are visible single cells and some are clustered and grouped unevenly. A pap smear is the typical screening procedure, but when a pap smear is combined with a test for human papillomavirus (hpv, the known cause of cervical cancers), screening every 5 years is acceptable for women aged 30 and above.
The addition of koh to the wet smear lyses the epithelial cells and makes the hyphae more apparent budding yeast may also be visualized on a wet smear (figure 4. Cytological examination and a smear on the flora as a rule, while examining smears stained by romanovsky’s method a doctor may reveal yeast-like fungi reddening of the head of a penis, spots, erosion, plaque are the symptoms of yeast infection serological methods and pcr for the diagnosis of yeast infection are practically not used. Explain similarities and dissimilarities course:-biology review site assignment help biology q1 a explain what you observed in a plaque smear wet mount, direct stained slide, and indirectly stained slide what were the differences c explains what you observed in your yeast wet mount, direct stained slide, and indirectly stained.